Fig. 4

Twist mediates miR-548c-reduced cancer cell proliferation, migration and invasion. RL95-2, HEC-1 and SKOV-3 cells were transfected with anti-548c, pre-548c, Twist siRNA or Twist cDNA vector lacking 3’-UTR as indicated, and were assayed for cell proliferation (a, b), migration (c, d) and invasion (e, f) (top). Western blot analysis of Twist protein levels (bottom). ^** P < 0.01