Fig. 1

SPAG9 expression is inversely correlated with miR-141 level in HCC. a The location of target sites of miR-141 in the SPAG9 3′-UTR is shown. b The protein level of SPAG9 in 10 paired HCCs (T) and non-cancerous hepatic tissues (N) were determined by Western blot analysis. (C and D) The expression levels of miR-141 were performed by real time-PCR in HCC tissues (c) and cell lines (d). e Western blot analysis of SPAG9 expression levels in human liver cell lines. Data are presented as the mean ± S.D. (*P < 0.05, n = 3)