Fig. 2

Validation of SPAG9 as the direct target of miR-141. a Wild-type (WT) and mutant (Mut) 3′-UTR of SPAG9 were cloned into a pmiR-RB-REPORT™ vector. b Dual-luciferase activity of the wild-type (WT) and mutant (Mut) SPAG9 3′-UTR reporter constructs in the presence of miR-141 or control miRNA (miR-NC). c MiR-141 expression levels were analyzed by real time-PCR in miR-141- or control miRNA- (miR-NC) transfected LM3 and Huh7 cells. d Western blot analysis of SPAG9 expression in LM3 and Huh7 cells transfected with miR-141 mimics or control miRNA. Data are presented as the mean ± S.D. (*P < 0.05, n = 3)