Fig. 4

MiR-141 inhibits HCC cell growth and metastasis by targeting SPAG9. a SPAG9 plasmid or control vector was transfected into miR-141-overexpression HCC cells. MTT assay showed that the restoration of SPAG9 in miR-141-transfected cells attenuated the inhibitory effects of miR-141 on cell proliferation, especially at 48 h and 72 h. b, c Matrigel invasion assay showed that restoration of SPAG9 reversed the inhibitory effects of miR-141 on the invasion of Huh7 cells. d, e Wound-healing assay showed that the restoration of SPAG9 increased the migration of miR-141-transfected Huh7 cells. Data are presented as the mean ± S.D. (*P < 0.05, n = 3). U.T: untreated HCC cells; miR-141 + Ctrl: miR-141 and control vector co-transfected HCC cells; miR-141 + SPAG9: miR-141 and SPAG9 plasmid co-transfected HCC cells