Fig. 6

DAB2, a predicted target gene of miR-106b, was inhibited by TGF-β1 in part via miR-106b. a Real-time PCR analysis of mRNA expression of miR-106b in HeLa cells with TGF-β1 treatment for 24 h. Data are mean ± SEM (n = 3). ^* P < 0.01 compared to control. b Western blot analysis of protein level of DAB2 in HeLa cells transfected with miR-106b inhibitor (anti-miR-106b) or miR-106b control inhibitor (anti-NC). Data are mean ± SEM (n = 3). *P < 0.05 compared to control. c Western blot analysis of DAB2 protein level in HeLa cells transfected with 100 nm miR-106b inhibitor (anti-miR-106b) for 24 h, then with or without TGF-β1 (5 ng/mL) for 24 h; GAPDH was an internal control. d Quantification of C.*P < 0.05