Fig. 2
Cell proliferation in vitro. a MGC-803, MGC-803-Sc and MGC-803-NRPlow cells were cultured for 7 days, and their viability measured. b Cellular expression of NRP-1, p27, cyclin E and CDK2 proteins was detected by immunoblotting. c-f MGC-803 and MGC-803-NRPlow cells were cultured for 4 days. c, d Cell proliferation was analyzed by a BrdU assay. c The percentage of BrdU-positive cells was plotted. d Representative images from the nuclei of proliferating cells stained green by BrdU, and the nuclei of all cells stained blue by DAPI. Magnification bar = 200 μm. e Cells were subjected to flow cytometry to measure cell cycle distribution. f The percentages of cells at the G1 phase were plotted. The density of each band was measured and normalized to β-actin. “*” (P < 0.05) and “**” (P < 0.001) indicate a significant difference from MGC-803 cells