Fig. 4

Tumorigenesis, metastasis and tumor growth in vivo. a, b MGC-803 and MGC-803-NRP^low cells were subcutaneously inoculated into the mice. a Tumor volumes were recorded. The number of tumor-bearing mice/ total number of mice are shown in brackets. b Tumors were weighed at the end of experiments. c, d The above cells were intravenously injected into the mice. The mice were killed 7 weeks later to harvest the lungs, which were sectioned and stained by hematoxylin/eosin (c), and weighed (d). e, f BGC823 tumors were established in mice, and injected with control Sc-shRNA or NRP-1 shRNA when they reached ~100 mm³ in volume. e Illustrated are representative tumor sections prepared 4 days following intratumoral injection. NRP-1 was immunostained brown by an anti-NRP-1 Ab. Magnification × 400. f The sizes of tumors were measured. “n” indicates the number of tumors. “*” indicates P < 0.05, and “**”, P < 0.001