Fig. 5

HOXA1 knockdown induces marked cell cycle arrest. a Flow cytometry results showing the cell phase distribution of GC cells. HOXA1 knockdown cells (the sh-HOXA1 group) show G1 phase arrest and a reduced number of cells in S phase compared to cells in the control groups. b The percentages of cells in each phase were quantified (cell phase distribution). c Proliferating SGC-7901 and BGC-823 cells were labeled with EdU and Hoechst33342. EdU staining is shown in red, and Hoechst33342 staining is shown in blue, an overlay of the EdU and Hoechst 33342 stained images are shown. Fewer EdU-positive cells were detected in the sh-HOXA1 group than the control groups, which is indicative of a reduction of the number of cells in S phase in the sh-HOXA1 group compared to that in the control. d The percentage of EdU-positive cells was quantified. e Western blot analysis of cyclin D1 expression in stable HOXA1 knockdown SGC-7901 and BGC-823 cell lines. (*P < 0.05, **P < 0.01, ***P < 0.001)