Fig. 4

MiR-302 decreased P-gp mRNA and protein expression not through targeting the 3′-UTRs of P-gp in MCF-7 and MCF-7/ADR cells. a qRT-PCR showing the mRNA expression of P-gp in MCF-7and MCF-7/ADR cells. b Western blot and densitometric analysis showing the protein expression of P-gp in MCF-7 cell and MCF-7/ADR cell. c Dual-luciferase assays showing repression of wild-type UTR (ABCB1-3′UTR-FULL) following transfection of miR-302 and individual mimics or negative control mimic (NC). d The stability assay of P-gp mRNA. MCF-7/ADR cells were harvested at 0, 2, 4 and 6 h after treatment with 2 μg/ml actinomycin residual mRNAs was detected by qRT-PCR. All graphs show means ± S.D. of three independent experiments