Fig. 5

miR-302a,b,c and d directly targeted the 3′UTR of MEKK1 mRNA with cooperative effects. a Validation of the miR-302 target site in the 3′UTR of MEKK1 mRNA. The putative miR-302 targeted sequence in the MEKK1 mRNA. b RNAhybrid predicts one binding site of miR-302 in the MEKK1 3'-UTRs. The binding energy was calculated c & d Dual-luciferase assays showing repression of wild-type UTR MEKK1-3′UTR-FULL (c) or mutant UTR MEKK1-3′UTR-MUT (d) following transfection of miR-302 mimics or negative control mimic (NC). All graphs show means ± S.D. of three independent experiments. (* P < 0.05 vs. NC;^# P < 0.05 vs. Single)