Fig. 2

IL-6/STAT3 signaling activation increased expression of DNMT1 and negatively regulated SOCS3 expression. a Protein expression levels of pSTAT3 and SOCS3 were analyzed in Aspc1 and Panc1 cells after IL-6 (100 ng/ml) and Bxpc3 and Capan2 cells after S31-201 (a STAT3 inhibitor, 10μM) treatment for 24 h using western blots. b mRNA expression levels of SOCS3 were analyzed in Aspc1 and Panc1 cells after IL-6 (100 ng/ml) and Bxpc3 and Capan2 cells after S31-201 (a STAT3 inhibitor, 10μM) treatment for 24 h using quantitative real-time PCR. Results were expressed as SOCS3/ACTIN. Gene expression measurements were performed in triplicate. Bars represented the mean ± SD. * p < 0.05, ** p < 0.01. c Protein expression levels of pSTAT3, STAT3, DNMT1 and DNMT3a were analyzed in Aspc1 and Panc1 cells after IL-6 (100 ng/ml) and Bxpc3 and Capan2 cells after S31-201 (a STAT3 inhibitor, 10μM) treatment for 24 h using western blots