Fig. 1

KDM5B expression is obviously up-regulated in HCC tissues and cell lines. a Expression profiles of a number of histone demethylase genes in a subset of HCC samples from public GEO Profiles database. b Validation of the aberrantly up-regulated KDM5B in 50 pairs of HCC and the corresponding adjacent non-cancerous liver (non-HCC) by quantitative real-time PCR. Expression of β-actin was used as an internal control and P value was calculated by student’s t test. (***, P < 0.001). c Representative results of up-regulation of KDM5B in 8 pairs of HCC (c) and the adjacent non-HCC liver tissue (N) by western blot. d The expression levels of KDM5B was determined by real-time PCR in 15 HCC cell lines as well as the normal human liver cell line L02 and normal adult liver tissue, with β-actin as an internal control