Fig. 1

Alignment and high similarity of mouse (top line) and human mesothelin protein sequences. Mouse mesothelin (625 amino acids) is predicted to have an amino terminal signal peptide, a furin cleavage site at position 298, and a carboxy-terminal glycosyl-phosphatidylinositol (GPI) linked anchor sequence. Signal peptide, N-linked glycosylation consensus sites, furin cleavage sequence, and GPI-anchor are underlined, and peptides used as the immunizing antigen for anti peptide antibody production are shaded