Fig. 8

Soft agar assay. A base layer of 1 ml of 0.5 % agar in RPMI was poured into wells in a 6 well plate and cooled to solidify. 2 ml of warm (40 °C) 0.2 % agarose/RPMI cell solution (25,000 cells per ml) were layered on top of each well. Cells (vector control or corresponding mesothelin-overexpressing clones) were incubated at 37 °C 5 % CO2 for 3 weeks. 500 ul of RPMI per well were added once per week. Wells were photographed macro-and microscopically for colony counting and measurement