CCR7 mediates the TNF-α-induced lymphatic metastasis of gallbladder cancer through the “ERK1/2 - AP-1” and “JNK - AP-1” pathways

Table 1 The primer sequences used in this study

Oligonucleotides	Sequences (5′ → 3′)^a	Length of products (bp)
CCR7 promoter cloning
−1055/+74F	ccg CTCGAG CAGCCCAAACATCCAACT	1129
−725/+74F	ccg CTCGAG GTGATGGTTGCATAACTCTG	799
−462/+74F	ccg CTCGAG GGAAAGCCATTTGGGACT	536
−243/+74F	ccg CTCGAG GGATCCTATGACCAGCGAC	317
−108/+74F	ccg CTCGAG TGGCTTCTCCGACAACTT	182
−1055/+74R	ccc AAGCTT CAGGGTCAGTCTGGGTGT
Site-directed mutagenesis
AP1mut1F	CTGTCACCCTCTGGCACCTTTCTTCTG
AP1mut1R	CAGAAGAAAGGTGCCAGAGGGTGACAG
AP1mut2F	CCTGCATGAGTTCAGGAGGGCTGGG
AP1mut2R	CCCAGCCCTCCTGAACTCATGCAGG
PCR for ChIP
AP1 ChIP-F	CACCTTTCTTCTGCCCCTTTATC	134
AP1 ChIP-R	TCAAGCCCCTTTTAAGTTGTCGG	134

^aLowercase letters represent the protective-bases; italic letters represent the restriction enzyme cutting site; underlined letters represent the bases of mutation

ISSN: 1756-9966