Fig. 3

SOX2 inhibition induced G1/S cell cycle arrest. a, and b The cell cycle was assayed by flow cytometry. The percentages of cell cycle phases were displayed in bar charts. siSOX2 transfection resulted in a significant increase of the cells proportion in the G0/G1 phases of A673 and RD-ES cells. c SOX2 inhibition amplified the expression of p21 and p27, and repressed cyclin-E, but had no effect on cyclin-D expression in Ewing’s sarcoma cells according to assayed by WB analyses. The data are representative and selected from one of three independent experiments