Fig. 1

Flow cytometry gating strategy for MRD assessment: representative analysis of a bone marrow sample. a CD38bright PC population, b CD45+ Lymphocytes (Ly), c surface membrane expression of CD19 and CD56 assessed on CD38bright PC, d cy-Ig Kappa/Lambda ratio on CD19+ PC (normal; ratio 1.9); e cy-Ig Kappa/Lambda ratio on CD56+ PC (clonal; ratio 0.01); f cy-Ig Kappa/Lambda ratio on CD19+ Ly utilized as internal control (normal; ratio 1.8); g cy-Ig Kappa/Lambda ratio on the PC and Ly populations; h populations hierarchy