Fig. 2
From: Targeted p53 activation by saRNA suppresses human bladder cancer cells growth and metastasis
dsP53-285 inhibits cells proliferation, clonegenesis and induces cell cycle arrest. T24 and EJ cells were transfected with 50 nM of the indicated dsRNAs for 72 h. Mock sample was transfected in the absence of dsRNAs. a Viable cells were measured from day 1 to 5 following transfection using the CellTiter 96® AQueous One Solution Cell Proliferation Assay kit. Results were plotted as OD values. b Representative photographs of colony formation assay. c Quantification of the cell colonies formation. d Representative photographs of cell cycle analysis. e Quantification of cell cycle distribution. f Photographs of tumors excised 28 days after inoculation of stably transfected cells EJ into nude mice. g Mean tumor volume measured by caliper on the indicated days. h Tumor weight of each nude mouse at the end of 28 days. * P < 0.05, ** P < 0.01 and *** P < 0.001 compared to dsControl or Lenti-dsControl group