Fig. 3

a MBIC disrupts mitotic spindle: HeLa cells expressing EGFP-α-tubulin, EGFP-CENP-A and histone H2B-mCherry were treated with DMSO (upper), MBIC (10 μM, middle), or nocodazole (2 μM, lower) and imaged at 15 min intervals. Time (min) is indicated in the upper left of each panel. For middle and lower panels, arrowheads mark the cell in the center at the first frame. Scale bar = 5 μm. b Effect of MBIC on tubulin polymerization: Tubulin polymerization assay was conducted using Tubulin polymerization assay kit (Cytoskeleton, Inc.). The plate was read using Infinite® 200 PRO—Tecan regulated on 96 well plate reader spectrophotometer. The plate was read at 340 nm in kinetic mode for two hours. Figure 3 shows curves of tubulin treated by paclitaxel (10 μM), nocodazole (10 μM), colchicine (10 μM), MBIC (10 μM) and untreated tubulin. Maximal velocity (Vmax) of each drug on tubulin polymerization was calculated