Fig. 5

a Effect of MBIC on nuclear morphology, membrane permeabilization, MMP (Δψm) and cytochrome c release: HeLa cells were seeded in 96-well plate and treated with two different concentrations of MBIC (0.2 and 0.4 μM) for 24 h. Cells were fixed and stained according to manufacturer protocol. Images were acquired using Cellomics HCS array scan reader (objective 20 ×). Figure a displays changes in HeLa cells DNA content (blue), cell permeability (green), MMP (red) and cytochrome c (cyan). MBIC induced a considerable elevation in membrane permeability and cytochrome c release and caused an extensive reduction in mitochondrial membrane potential. b, c & d shows bar chart representing changes in membrane permeability, mitochondrial membrane potential, and cytochrome c in a dose-dependent manner. Data were mean ± SD of three independent experiments. All the treatment groups were compared with control. “*” indicates statistically significant at P < 0.05