Fig. 6

PPP2R2D is a direct downstream target gene of miR-133b. a The schematic diagram shows the PPP2R2D 3’UTR (2R2D-3’UTR) regions containing the binding site for miR-133b. b Predicted structure and free energy of binding between miR-133b (green) and 2R2D-3’UTR (red). c The miR-133b expression in normal hepatic cell lines and HCC cell lines. *P < 0.01 as compared with L02 cells; ^# P < 0.01 as compared with QSG7701 cells. d Pearson’s correlation analysis of miR-133b and PPP2R2D mRNA levels among HCC cell lines. e Spearman’s correlation analysis of miR-133b and PPP2R2D mRNA levels in HCC xenograft tumors. f Luciferase activity of pGL3c-2R2D-3’UTR reporter gene in HepG2 cells treated with or without 2.5 μg/ml cDDP for 6 h. The data shown are the mean ± SD of three parallel samples, *P < 0.01. g miR-133b and PPP2R2D mRNA levels in HepG2 cells treated with 2.5 μg/ml cDDP for 0, 3, 6, or 12 h or treated with 0, 1, 2.5, or 4 μg/ml cDDP for 12 h. *P < 0.01 as compared with cDDP-untreated group (Ctrl)