Fig. 2

The regulatory effect of PHB on ERK pathway activation in GBCs. a PHB protein expression and distribution in NOZ and SGC-996 cells were detected by immunofluorescence staining. b PHB, p-ERK and ERK expression levels in NOZ and SGC-996 cells were quantified by western blotting analysis. β-actin was used as the loading control. c PHB overexpression was frequently accompanied by upregulation of p-ERK expression in GBC tissue specimens. d PHB, p-ERK and ERK expression levels in Lv-sh NC and Lv-sh PHB GBC cells were determined by western blotting. e and f The NOZ and SGC996 cells overexpressing PHB were treated with PD0325901 (20 nmol) or vehicle for 24 h followed by the analyses of western blotting and gelatin zymography assays