Fig. 3

Increased Bax/Bcl-2 ratio and decreased Ki67 protein expression by GT, Q and Doc in combination. Eight tumor samples were randomly selected from each group for this analysis. The protein expression of Bax and Bcl-2 in tumor tissues was detected by Western blot. Three representative protein images from each group were demonstrated (a). The ratios of Bax to Bcl-2 are presented as mean ± SD (b). A section of tumor tissue was formalin-fixed and paraffin-embedded for tissue microarray and immunohistochemical detection. Slides were cut from the arrays and incubated with primary antibodies for detection of Ki67 (c). The slides were counterstained with hematoxylin. Nuclei were stained in blue and Ki67 in brown color. The positive rates of Ki67 nuclear staining are presented as mean ± SD (d). GT, green tea; Q, quercetin; Doc 5, docetaxel at 5 mg/kg iv. Columns with different letters represent significant difference between treatments (P < 0.05)