Fig. 6

Validation of intra-tumor oncolytic adenoviral replication and the expression patterns of the encoded TRAIL and IL-12 transgenes. Three days post-treatment of HCC-bearing mice with PBS, Ad-ΔB, or Ad-ΔB/TRAIL+Ad-ΔB/IL-12 (at a dosage regimen of 1 × 10¹⁰ VP in 200 μL PBS of each virus; repeated three times every other day), the mice were euthanized under general anesthesia and their liver tumor tissues were harvested and investigated to validate intratumor viral replication and expression of TRAIL and IL-12 transgenes. a and b represents intra-tumor TRAIL expression at its mRNA and protein level, respectively, and (c) and (d) represents intra-tumor IL-12 expression at its mRNA and protein level, respectively. Data are represented as Mean ± SE. e represents the expression of Ad E1A protein (an indicator of viral production) by western blot assay