Fig. 4
No synergistic effect on glioblastoma cell invasion was observed in the combination of isoform-selective PI3K inhibitors and SP600125. Boyden chamber invasion assay of U-87 MG cells treated with PIK-75 (0.1 μM), TGX-221 (20 μM) or CAL-101 (10 μM) alone and combined with SP600125 (20 μM) for 24 h. a Representative photographs showing the invasive cells that had passed through matrigel to the lower surface of the membrane at 100× magnification. Invaded cells from 5 representative fields were counted. b-d Number of invasive cells was significantly decreased by PIK-75 and SP600125, and all the combinations did not enhanced the inhibitory effect (n = 3; p values were determined by One-way ANOVA and Post Hoc multiple comparison Tukey HSD test. *: p <0.05; **: p <0.01)