Fig. 2

RNF8 regulates EMT in breast cancer cells. a Overexpression of RNF8 induced EMT in MCF7. MCF7 cells were infected with TG006 (empty vector with GFP) or TG006-RNF8 virus, the morphological change and the expression of E-Cadherin were examined 5 days later by IMC (upper) or by indirect immunofluorescence (bottom), using anti-E-cadherin antibody (red, E-cadherin; blue, DAPI). b Expression of the indicated EMT markers in the infected MCF-7 cells was detected by Western blotting. β-actin was used as an internal standard. c MDA-MB-231 cell lines were transiently transfected with 20nM siRNA (siControl or siRNF8-1), morphology of the transfected cells were examined 72 h later by IMC (upper), and the expression of mesenchymal marker was detected by indirect immunofluorescence (bottom) using anti-vimentin antibody (red, vimentin; blue, DAPI). d Expression of the indicated EMT markers in the infected MDA-MB-231 cells was detected by Western blotting. β-actin was used as an internal control. Magnification, 40 ×; bar: 5 μm