Fig. 2

PIK3CA is vital for the proliferation, migration, invasion and clone formation of GBC in vitro. a, b GBC-SD and NOZ cells treated with CON, NC and siRNA were evaluated via a CCK8 cell viability assay. Cell proliferation was represented as cell quantity. Data are presented as mean ± SD (n = 5). c, d Migration assays of the GBC-SD and NOZ cells treated with CON, NC and siRNA were evaluated with uncoated transwells. Representative stained images of migrated cells and the numbers of migrated cells (mean ± SD, n = 5) are shown. e, f Invasion assays of the GBC-SD and NOZ cells treated with CON, NC and siRNA were evaluated with Matrigel-coated transwells. Representative images of stained invaded cells and the numbers of invaded cells (mean ± SD, n = 5) are shown. g, h GBC-SD and NOZ cells were treated with CON, NC and siRNA and allowed to form colonies. Representative stained images and the colony numbers (mean ± SD, n = 3) are shown