Fig. 4
From: SYNJ2BP inhibits tumor growth and metastasis by activating DLL4 pathway in hepatocellular carcinoma
Synaptojanin 2 Binding Protein (SYNJ2BP) inhibits proliferation, invasion and migration of hepatocellular carcinoma (HCC) cells. a MTT assay was carried out to investigate proliferation ability in the above cells. Obvious increases of proliferation were observed in Hep3BshSYNJ2BP and HCCLM3NC cells compared with Hep3BNC and HCCLM3SYNJ2BP cells respectively. (P < 0.05) b Colony formation assay was also conducted to measure proliferation capacity of the above HCC cells. The results showed us that more colonies were formed in Hep3BshSYNJ2BP (192 ± 19) and HCCLM3NC (290 ± 28) cells as compared with Hep3BNC (109 ± 21) and HCCLM3SYNJ2BP (160 ± 17) cells. (P < 0.05) c Transwell assay showed that more Hep3BshSYNJ2BP (81 ± 8) and HCCLM3NC (103 ± 10)cells passed through the matrigel than Hep3BNC (23 ± 3) and HCCLM3SYNJ2BP (39 ± 2)cells. (P < 0.05) d For wound healing assay, the results showed that the closure of Hep3BshSYNJ2BP (82 %) and HCCLM3NC (76 %) cells was significantly faster than that of Hep3BNC (31 %) and HCCLM3SYNJ2BP (21 %) cells (P < 0.01). Original magnification: 200× for (c); 100× for (d); b pictures were captured by a camera. *P < 0.05