Fig. 5

Silencing of Nestin on chemosensitivity in vivo. a The mice were imaged by Fluorescence Molecular Tomography (FMT-4000, PerkinElmer). b Images of primary tumors in Nestin silenced groups, corresponding controls groups, including intraperitoneal injection of 5-FU groups. c Primary tumor size was calculated by TrueQuant™ (the Quantitative Tomography In Vivo Imaging Software). The tumors in mice that received 5-FU treatment and stable Nestin-shRNA transfection were smaller than those either treated with 5-FU or transfection alone (all P < 0.001). d The mean fluorescence intensity (Total MMPs/Tumor Size) of tumors in Si-Nestin group were smaller than those in Si-Control group (P < 0.001). e Representative HE and IHC staining of proteins (Nestin, E-cadherin, Vimentin and β-Catenin) in tumor tissues of nude mice. (Original magnification: ×400)