Fig. 4
Anti-cancer efficacy of a combined treatment regime composed of 1-MNA or 1,4-DMP and cyclophosphamide in the 4T1 cancer model. For ease of interpretation, graphs are presented for the two therapeutic regimes separately. Tumor weight estimated on the 29th day of the experiment for mice treated with a 1-MNA and cyclophosphamide (CP) or b 1,4-DMP and cyclophosphamide (CP) both presented with relevant controls; data are presented as median values  ± min/max. c Tumor growth inhibition (TGI) values observed for combined therapeutic regimes presented in reference to TGI of cyclophosphamide alone. In Fig. 4C, days of the drug administrations are indicated with arrows (1-MNA and 1,4-DMP - solid arrow, cyclophosphamide – gray arrows). The number of lung metastases in mice receiving d 1-MNA and cyclophosphamide (CP) or g 1,4-DMP and cyclophosphamide (CP) presented with corresponding controls; data are presented as raw values with medians. TXB2 plasma levels in mice receiving e 1-MNA and cyclophosphamide (CP) or h 1,4-DMP and cyclophosphamide (CP); data are presented as median values ± min/max. vWF plasma levels in mice receiving f 1-MNA and cyclophosphamide (CP) or i 1,4-DMP and cyclophosphamide (CP); data are presented as median values ± min/max. j The images of bands obtained in Western blot analysis of tissue samples isolated from the animals assigned to treatment with: (a) control, (b) cyclophosphamide (CP), (c) 1-MNA, (d) 1-MNA and cyclophosphamide (CP), (e) 1,4-DMP, (f) 1,4-DMP and cyclophosphamide (CP). k E-cadherin : N-cadherin expression ratios in the samples of tumor tissue collected on the last day of the experiment. The total cellular content of E-cadherin (comprising protein characterized by the molecular weight of 100 and 130 kDa) and N-cadherin was first normalized to the content of β-actin and then used to determine E-cadherin to N-cadherin expression ratios that are presented on the graph as median values with min/max