Fig. 3

Irradiation upregulated FasL expression in SMMC7721 cells and MHCC97H cells. a Protein levels of FasL in HCC cells were determined after be irradiated with doses of 0, 2, 4, 6, 8Gy and incubated for 24 h. b FasL expression was detected in HCC cells after be irradiated with 4 Gy and incubated for 12, 24, and 48 h, respectively, by Western Blot. GAPDH was used as a loading control. c Increased expression of FasL was confirmed by immunofluorescent staining inHCC cells after be irradiated with 4Gy and incubated for 24 h