Table 1 Primers for PCR assay
Gene name | Sequence (5’ → 3’) |
|---|---|
Reverse transcription PCR primers | Â |
 CLDN6 |  |
  Sense | CACTGCCACTTCTGGATGG |
  Antisense | CAGTGCAGCTCCTTCAACCT |
 β-actin |  |
  Sense | CCACTGCGTCGCGGGG |
  Antisense | GGCAGCCAGCTCAGCCATG |
 GAPDH |  |
  Sense | TGTTGCCATCAATGACCCCTT |
  Antisense | CTCCACGACGTACTCAGCG |
Methylation-Specific PCR primers | Â |
 Unmethylated DNA |  |
  Sense | TGGATGTTTGTTAGTTTGAGGT |
  Antisense | ATAACCACAACCCAAATTCACA |
 Methylated DNA |  |
  Sense | ACGTTTGTTAGTTCGAGGC |
  Antisense | ATAACCGCAACCCGAATTC |
ChIP primers | Â |
 CLDN6 |  |
  Sense | CCCATCCCCACGCACGCTT |
  Antisense | GCCACCTGGATGGGCGAGTC |
Nuclease Accessibility primers | Â |
 Primer1 |  |
  Sense | TCTCCCCAAGGTGGTCTGCT |
  Antisense | CCTTTTCAAATCCAGGTTCGGC |
 Primer2 |  |
  Sense | GGCGGTGGTCCAGTGACGT |
  Antisense | TAAAGGGAATAG AGGAAGCGTCC |
 Primer3 |  |
  Sense | AGTCTCCCCAAGGTGGTCTGC |
  Antisense | CCTTTTCAAATCCAGGTTCGGC |
 Primer4 |  |
  Sense | AGGAATTTCCCTTATCTCCTTCGC |
  Antisense | CCCATCCCCACGCACGCTT |
 Primer5 |  |
  Sense | GCCACCTGGATGGGCGAGTC |
  Antisense | GAGGGTTCCCAATTTGCGGG |
 Primer6 |  |
  Sense | CAGGGACAA AGACTCCGAAACC |
  Antisense | CTGGAACGCCGGTGACGT |
 Primer7 |  |
  Sense | CTCCCCAAGGTGGTCTGCT |
  Antisense | TCAAATCCAGGTTCGGCATGTTA |
 Primer8 |  |
  Sense | CCACTGCAGCTATAGAACCCCTCCA |
  Antisense | TTCTTTCCCCGTCCCCG |