Fig. 2

CD11c⁺ CD8⁺ T cells show anti-tumor phenotypes in response to Poly(I:C). a Mice loaded with EG7 tumor were treated with OVA or OVA + Poly(I:C) at day 9. Six days later, spleens and DLN were harvested and the proportions of IFN-γ⁺ CD8⁺ T cells were evaluated on flow cytometer. b CD11c⁻ CD8⁺ T cells and CD11c⁺ CD8⁺ T cells were isolated from splenocytes by FACS sorting at day 15. Isolated cells were co-cultured with ⁵¹Cr-laveled EG7 or EL4 (E/T = 40) for 4 h. Then, the cytotoxicity against EG7 and EL4 was measured by ⁵¹Cr-release assay. c CD11c⁻ CD8⁺ T cells and CD11c⁺ CD8⁺ T cells were isolated from spleens by FACS sorting at day 15. The gene expression levels were measured by quantitative PCR. Error bars show ± SEM; n = 4 to 5 per group (a) and ± SD (b, c). Student’s t-test (a) and Kluskal-Wallis test with Dunn’s multiple comparison test (b) were performed to analyze statistical significance. * p < 0.05. The results are one of the two independent experiments