Fig. 1

CPS induces cell death in mutp53-expressing cells in a p53-dependent manner. a U373 and SKBR3 cells were plated at subconfluence and the day after treated with CPS (100 and 200 μM). Twenty-four hours later, the percentage of dead cells was scored by trypan blue staining. Error bars show standard deviation. b H1299 cells were transiently transfected with pcDNA3-p53R175H (0.1 μg), pcDNA3-R273H (0.1 μg) and control pcDNA3 vectors and the day after transfection treated with CPS (200 μM) for 24 h. Cell death measurements were assayed by Tunel assay. The results are the mean of three independent experiments performed in triplicate ± SD. *p = 0.001. c U373 and SKBR3 cells were treated with CPS (200 μM) for 16 and 24 h. Equal amount of total cell extracts was analysed by western immunoblotting with anti-PARP antibody; the cleaved form is indicated by the arrow. Anti-β-actin was used as protein loading control