Fig. 4

HSP70-2 ablation inhibits cellular motility of breast cancer cells. a Phase contrast microscopy images show difference in number of MCF7 and MDA-MB-231 cells migrating through the transwell-insert membrane when transfected with shRNA3, shRNA4 compared to NC shRNA. b Histogram demonstrates significant reduction in number of migratory MCF7 and MDA-MB-231 cells transfected with shRNA3 and shRNA4 compared to NC shRNA. c Phase contrast microscopy images depict reduced number of MCF7 and MDA-MB-231 cells invading through matrigel coated transwell insert membrane when transfected with shRNA3 and shRNA4 compared to NC shRNA. d Histogram shows significantly reduced number of shRNA3 and shRNA4 transfected MCF7 and MDA-MB-231 cells invading through insert as compared to NC shRNA. *P < 0.05. Data are represented as mean ± SEM. e SEM images show difference in the number of MCF7 (first panel) and MDA-MB-231 (second panel) cells migrating through transwell insert membrane when transfected with shRNA3 and shRNA4 compared to NC shRNA in migration. Magnification: ×700, WD = 6 mm, EHT = 20.00 kV. Third panel shows single MDA-MB-231 cell migrating through the transwell insert membrane. Magnification: ×4000, WD = 6 mm, EHT = 20.00 kV. f SEM images depict difference in the number of MCF7 (first panel) and MDA-MB-231 (second panel) cells invading through transwell insert membrane when transfected with shRNA3 and shRNA4 compared to NC shRNA. Magnification: ×700, WD = 6 mm, EHT = 20.00 kV. Third panel shows single MDA-MB-231 cell invading through the transwell insert membrane. Magnification: ×4000, WD = 6 mm, EHT = 20.00 kV. The three independent experiments were carried out in triplicates. g Western blot analysis demonstrates up-regulation of epithelial marker (E-Cadherin) and down-regulation of mesenchymal markers (N-Cadherin, P-Cadherin, MMP2, MMP3, MMP9, SLUG, SNAIL, TWIST, SMA, Vimentin) in HSP70-2 depleted MDA-MB-231 cells