Fig. 3
From: Non-benzoquinone geldanamycin analogs trigger various forms of death in human breast cancer cells
17-DR induced apoptosis in MDA-MB-231 cells that is dependent of caspase activity. a Whole-cell lysates from the MDA-MB-231 cells treated with different concentrations of 17-DR, or treated with 200 μM 17-DR for different lengths of time, were subjected to western blot analysis. b The expression of caspase-3, −8 and PARP. 17-DR increased their activation at 24 h. c Analysis of cell viability by MTT assay, following treatment with 17-DR with or without 1 h pre-treatment with Nec-1 or z-VAD-fmk. *P < 0.05. d Annexin V-FITC/PI analysis following treatment with 17-DR alone or with Nec-1 pre-treatment