Fig. 5
From: Non-benzoquinone geldanamycin analogs trigger various forms of death in human breast cancer cells
Knockdown of RIPs with siRNA protected against DHQ3-induced necroptosis in MDA-MB-231 cells. a, d MDA-MB-231 cells were transfected with control, or RIP1 or RIP3 siRNA, and after 48 h, whole-cell lysates were subjected to western blot analysis. b, e After 24 h transfection with RIP1/RIP3 siRNA, cells were treated with 200 μM of analogs for another 24 h, with or without 1 h pre-treatment with Nec-1 and z-VAD-fmk. Cell viability was measured by MTT assay. *P < 0.05. c, f Similarly to b, MDA-MB-231 cells were analyzed by Annexin V-FITC/PI assay