Fig. 2From: Shock wave-induced ATP release from osteosarcoma U2OS cells promotes cellular uptake and cytotoxicity of methotrexateShock waves enhanced the uptake of Calcein and LY in U2OS cells. a-b, U2OS cells were stained with Calcein (green) or PI (red) and treated with shock waves as described. c-d, U2OS cells were treated with LY and subjected to shock waves as indicated. LY (green) or PI (red) influx was measured. e Cells were treated as described and LY and Calcein influx was assessed. Data are mens ± SD, n = 3, *p < 0.05,Student’s t-test. **In calcein + S1: addition of calcein prior to shock wave treatment; in S1 + calcein: shock wave treatment prior to addition of calcein. Same for LYBack to article page