Fig. 9

Cytokines released by autologous CD3 + T-cells stimulated with tumor antigen pulsed dendritic cell. Magnetic assay cell sorting (MACS)-enriched CD3⁺T cells were stimulated with autologous DCs pulsed with NP-containing tumor lysate as well as tumor lysate alone and untreated control-NPs for 5 days and IFN-γ and IL-4 production were measured using commercially available ELISA kit. Triplicate data obtained from three patients were averaged and expressed as mean ± S.D (n = 3). a cytokines produced by T cells, b IFN-γ:IL-4 ratio