Fig. 1

Expression of Gal-1 in CAFs and expression of Gli1 in human GC cells. a Western blot showing the expression of Gal-1 in NGFs, CAFs, Over cells, Down cells and their respective conditioned medium (CM). b Quantification of Gal-1 expression relative to β-actin. *P < 0.05 vs. NGFs, **P < 0.01 vs. NGFs. c Expression of Gal-1 in supernatants was quantified using ELISA. Gal-1 showed increased expression in supernatant from Over cells and reduced expression in supernatant from Down cells. d The expression of Gli1 at the protein level in the GC cell lines was determined by western blotting. e qRT-PCR analysis of the GC cell lines revealed high levels of Gli1 mRNA in all tested cell lines. Expression levels relative to MGC-803 cells are shown. The expression of each target gene was normalized to the reference gene GAPDH. The bars represent the mean of three independent experiments ± SD