Fig. 3

Downregulation of Gli1 abrogates Gal-1-induced migration, invasion and EMT of human GC cells. a The expression of Gli1 protein in MGC-803 cells after treatment of Over-CM was evaluated by western blotting (left) and qRT-PCR (right). Gli1 expression was upregulated in a time dependent manner at both the protein level and the mRNA level. * P < 0.05 when compared with expression at 0 h. b Silencing of Gli1 expression by siRNA at 48 h following transfection was confirmed by western blotting (left) and qRT-PCR (right). *P < 0.05 when compared with the si-Control. c Gal-1-induced GC cell migratory ability was impaired by Gli1 knockdown. Representative images of GC cells treated for 48 h with CM from Over cells and Down cells exhibited significantly enhanced migratory abilities. These effects were abolished by Gli1 knockdown. *P < 0.05. d The effect of Gal-1 on cell invasion was impaired by Gli1 knockdown (original magnification × 100). *P < 0.05 when compared to control cells without treatment with Over-CM. e Loss of Gli1 expression reverses the effects of Gal-1-induced EMT in GC cells. *P < 0.05. These results are presented as the mean ± SD of three independent experiments