Fig. 4

β1 integrin is necessary for Gal-1-induced effects in GC cell migration, invasion, and EMT. a The silencing of β1-integrin expression by siRNA at 48 h was confirmed using western blotting (left) and qRT-PCR (right). *P < 0.05 when compared with the si-Control. b The expression of EMT-related molecules (E-cadherin, N-cadherin, vimentin, and snail), invasion-related molecules (MMP-9), key molecules in the Hh pathway (SHH, SMO, and Gli1) and β1 integrin were analyzed by western blotting (left). The mRNA expression levels were determined by qRT-PCR (right). *P < 0.05. The results are presented as the mean ± SD of three independent experiments. c MGC-803 cells were labelled with fluorescein-conjugated Gli1 specific antibody (green) following treatment with CM from Over (magnification × 200). The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). d The invasion-promoting effects of Gal-1 were abolished by Gli1 knockdown. The number of invading cells was quantified under a light microscope by counting six random fields at a magnification of 200×. *P < 0.05. The data represent the results of three independent experiments