Fig. 4

Knockdown of WT1 resembles the anti-metastasis activity by miR-193a. A549 and H1299 cells were transfected with specific shRNA for WT1 or nonspecific shRNA (sh-NC). a and b WT1 protein and mRNA levels were detected in A549 and H1299 cells transfected with sh-NC or sh-WT1. *P < 0.01 versus sh-NC. c and d Transwell migration (c) and invasion (d) assays were performed in A549 and H1299 cells transfected with sh-NC or sh-WT1. Scale bar = 50 μm. *P < 0.01 versus sh-NC. e Colony formation was performed by methylene blue staining in A549 and H1299 cells transfected with sh-NC or sh-WT1. *P < 0.01 versus sh-NC. f and g Cell proliferation was detected by CCK-8 assay in A549 (f) and H1299 (g) cells transfected with sh-NC or sh-WT1 for 24, 48, and 72 h. *P < 0.01 versus sh-NC