Fig. 3

miR-589-5p directly targets MAP3K8. (a) The potential binding sites for miR-589-5p in the MAP3K8 mRNA 3ˈ-UTR were determined with in silico predictions. The underlined bases were mutated to AAGA in the mutant plasmid. (b) The expression of MAP3K8 in CD90⁺ and CD90^- cells sorted from MHCC97H and MHCC97L cells measured by qRT-PCR and Western blot. (c) Luciferase activities of MAP3K8 wild-type (Wild) and mutant reporter plasmids in 293 T cells co-transfected with miR-control or miR-589-5p. (d) CD90⁺ MHCC97H and MHCC97L cells were transfected with miR-589-5p mimics for 24 hours, and the level of MAP3K8 expression was measured by qRT-PCR and Western blot. All data are representative of three independent experiments and are shown as mean ± SEM (n = 3)