Fig. 3
From: Hyaluronan synthase 2 expressed by cancer-associated fibroblasts promotes oral cancer invasion
CAFs promoted migration, invasion and EMT of Cal27 cells. a Representative images of CAF-CM promoting Cal27 cell migration compared to control and NFs-CM for 36 h incubation. b Representative images of Cal27 cell proliferation by BrdU incorporation assay after treated with CAFs-CM or NFs-CM. c The cells invading the filters coated with the Matrigel after 36 h (upper panel); and the quantification of the cell invasion in five randomly chosen fields (bottom panel). d The expression of EMT-associated genes E-cad, Vimentin, Twist, and Snail was measured by real-time PCR using SYBR Green. e The expression of E-cad and Vimentin was measured using western blotting. *p < 0.05; # p > 0.05 in (c) and (d). CM: conditioned medium