Fig. 4
From: Hyaluronan synthase 2 expressed by cancer-associated fibroblasts promotes oral cancer invasion
Knocking down HAS2 in CAFs reduced the migration, invasion and EMT of the Cal27 cells. The expression of HAS2 was evaluated in CAFs that had been transfected with a scrambled siRNA or an HAS2-targeted siRNA by western blotting (a) and immunofluorescence (b). Representative images of the migration (c) and invasion (d) of the Cal27 cells that were cultured with CM from CAFs, CAFs with a scrambled siRNA or CAFs with HAS2-targeted siRNA for 36 h. e The expression of EMT-associated genes was measured by real-time PCR using SYBR Green. f The expression of E-cad and Vimentin was analyzed using western blotting. *p < 0.05; # p > 0.05 in (d) and (e). Bars in (b): 100 μm. siRNA: short interfering RNA; CM: conditioned medium