Fig. 5
From: Hyaluronan synthase 2 expressed by cancer-associated fibroblasts promotes oral cancer invasion
Treatment of CAFs with 4-MU reduced the migration, invasion and EMT of the Cal27 cells. a The viability of CAFs after 48 h of treatment with various concentration of 4-MU assessed as the OD following the MTT assay. b The levels of the HAS isoforms in CAFs after 48 h of treatment with various concentration of 4-MU were measured by real-time PCR using SYBR Green. and shown as the average 2-ΔΔCT values. c The expression of HAS2 in CAFs cultured with various concentrations of 4-MU was determined by western blotting. d Representative images of the migration (d) and invasion (e) of Cal27 cells cultured with serum-free medium, CM from CAFs, or CM from CAFs that had been treated with 0.4 mM 4-MU. f The expression of EMT-associated genes was measured by real-time PCR using SYBR Green. g The expression of E-cad and Vimentin was examined by western blotting. *p < 0.05; # p > 0.05 in (a), (e) and (f). 4-MU: 4-Methylumbelliferone; CM: conditioned medium