Fig. 7
TY-011 antagonizes the angiogenesis. a Representative images of tumor tissues. b The tumors tissue were sectioned and subjected for immunohistochemistry of CD31 (angiogenesis marker) staining. White arrow means blood vessel. Representative images were shown. Scale bar, 50 μm. c The HTRF® KinEASE™-TK kit was used to measure inhibition of VEGFR2 by TY-011. The inhibition rates were determined in reference to the control. Data presented are the mean ± SD of three independent experiments. d Growth inhibition effects of TY-011 on HUVECs in the presence or absence of VEGF were determined by CCK-8 assay. Data presented are the mean ± SD of three independent experiments. e HUVECs grown on MatrigelTM were treated with various concentration of TY-011 or equivalent DMSO for 6 h. Representative images from three independent assays are shown. f Quantitation of tube formation in (e). Data presented are the mean ± SD of three independent experiments. g HUVECs cells were treated with various concentration of TY-011 for 6 h, and growth inhibition was determined by CCK-8 assay