Fig. 4
Overexpression of lnc-GNAT1-1 promoted proliferation, colony formation, cell motility, migration and invasion of LoVo cells. a LoVo cells were transiently transfected with lnc-GNAT1-1 expression plasmid, and the efficiency of lnc-GNAT1-1 overexpression was verified by real-time PCR, with the fold change of nearly 8 times. b CCK8 assay showed that lnc-GNAT1-1 overexpressed cells exhibited a remarkably decreased proliferation rate. c Colony formation assay showed that overexpression of lnc-GNAT1-1 decreased the formed colonies of LoVo cells. d & e Flow cytometry analysis showed that compared with control cells, overexpression of lnc-GNAT1-1 could induce cell apoptosis (13.71 ± 0.12%, 6.96 ± 0.12%, respectively, P < 0.001) and hinder cell cycle (G0/G1%: 46.54 ± 0.29% vs. 38.92 ± 0.28%, respectively, P < 0.001). f Wound healing assay showed that overexpression of lnc-GNAT1-1 could inhibit the mobility of LoVo cell (Magnification × 100). g Transwell migration (upper) and invasion (lower) assays showed that overexpression of lnc-GNAT1-1 could decrease the migration and invasion abilities of LoVo cells (Magnification × 100)