Fig. 4

CAPZA1 expression controls HCC EMT. a, b Following CAPZA1 siRNA treatment, western blotting was used to detect the expression of EMT-related markers in HepG2 cells, and ImageJ software was used to measure immune reactivity. The epithelial marker E-cadherin was down-regulated, and the mesenchymal markers N-cadherin and Vimentin were up-regulated following CAPZA1 down regulated. c Following CAPZA1 siRNA treatment, qPCR was used to detect the mRNA expression of EMT-related markers in HepG2 cells. The results were consistent with the western blots in a and b. d, e Following CAPZA1 overexpression, western blotting was used to detect the expression of EMT-related markers in MHCC97H cells, and ImageJ software was used to measure immune reactivity. The epithelial marker E-cadherin was up-regulated, and the mesenchymal markers N-cadherin and Vimentin were down-regulated following CAPZA1 overexpression. f Following CAPZA1 overexpression, qPCR was used to detect the mRNA expression of EMT-related markers in MHCC97H cells. The results were consistent with the western blots in D and E. g EMT transcription factors were detected. Following treatment of HepG2 cells with CAPZA1 siRNA, Snail1 and ZEB1 expression was up-regulated. Following CAPZA1 overexpression in MHCC97H cells, expression of Snail1 and ZEB1 was down-regulated. h Immunoprecipitation was performed to validate interaction between CAPZA1 and F-actin. The result showed that CAPZA1 and actin can pull each other down